Gene Cloning Research Papers

Gene Cloning Research Papers-75
The reasons for cloning are more complex than simply producing identical animals, and in this article we consider those reasons and the results that have been obtained with the procedure.

The reasons for cloning are more complex than simply producing identical animals, and in this article we consider those reasons and the results that have been obtained with the procedure.We place cloning in the historical context of developmental biology and review results obtained with the procedure.Dr Wulff's lab has also pioneered the technique of speed breeding which uses enhanced LED lighting to fast track genetic improvements in crops.

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"This is the culmination of a dream, the result of many year's work.

Our results demonstrate that Ag Ren Seq is a robust protocol for rapidly discovering resistance genes from a genetically diverse panel of a wild crop relative," he says.

The population contains a wealth of disease fighting genetic diversity which has been bred out of elite domesticated varieties.

The team inoculated the population of the wild relative with the stem rust pathogen and screened the plants to identify those resistant and susceptible to the disease.

"We have found a way to scan the genome of a wild relative of a crop plant and pick out the resistance genes we need: and we can do it in record time.

This used to be a process that took ten or 15 years and was like searching for a needle in a haystack.

It enables researchers to search a genetic "library" of resistance genes discovered in wild relatives of modern crops so they can rapidly identify sequences associated with disease fighting capability.

From there researchers can use laboratory techniques to clone the genes and introduce them into elite varieties of domestic crops to protect them against pathogens and pests such as rusts, powdery mildew and Hessian fly.

Now we can clone these genes in a matter of months and for thousands of pounds instead of millions." The research published today in the journal reveals that Ag Ren Seq has been successfully trialled in a wild relative of wheat - with researchers identifying and cloning four resistance genes for the devastating stem rust pathogen in the space of months.

This process would easily take a decade using conventional means.

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